Design of a Bioreductively-Activated Fluorescent pH Probe for Tumor Hypoxia Imaging

• Nakata, Eiji

• Yukimachi, Yoshihiro

• Abe, Chiaki

• Uto, Yoshihiro

• Hori, Hitoshi

書誌事項

タイトル別名

• 低酸素細胞特異的な蛍光イメージング剤の開発

抄録

We have designed and evaluated UTX-12 as a novel fluorescent pH probe for tumor hypoxia imaging. UTX-12 consists of a p-nitro benzyl moiety, which is a latent hypoxia-selective leaving group activated by nitro reduction, directly linked to SNARF. Although UTX-12 itself is colorless and non-fluorescent in aqueous solution, nitro reduction triggers the release of SNARF which has well-characterized long wavelength absorption and fluorescence that is sensitive to pH. The resultant SNARF, released intracellularly by enzymatic reduction of UTX-12, allows measurement of pH by pH-dependent dual emission shifts. UTX-12 showed clear differences in fluorescence behavior between hypoxic and aerobic conditions in liver microsomes and inside V79 cells. These data are confirmation that UTX-12 is biologically reduced inside tumor cells and the released SNARF should monitor intracellular pH of tumor cells selectively with reduced background signal.

収録刊行物

• 徳島大学大学院ソシオテクノサイエンス研究部研究報告

  徳島大学大学院ソシオテクノサイエンス研究部研究報告 55 2010

キーワード

• Fluorescent pH probe
• Tumor hypoxia
• Fluorescent imaging
• Bioreductive activation
• Nitro reduction

詳細情報

• CRID: 1050282812440437248
• NII論文ID: 120002932248
• NII書誌ID: AA12214889
• ISSN: 21859094
• Web Site: https://repo.lib.tokushima-u.ac.jp/74502
• 本文言語コード: en
• 資料種別: departmental bulletin paper
• データソース種別:
  • IRDB
  • CiNii Articles