A more efficient method to generate integration-free human iPS cells.

• Okita, Keisuke

• Matsumura, Yasuko

• Sato, Yoshiko

• Okada, Aki

• Morizane, Asuka

• Okamoto, Satoshi

• Hong, Hyenjong

• Nakagawa, Masato

• Tanabe, Koji

• Tezuka, Ken-Ichi

• Shibata, Toshiyuki

• Kunisada, Takahiro

• Takahashi, Masayo

• Takahashi, Jun

• Saji, Hiroh

• Yamanaka, Shinya

Abstract

We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

Journal

• Nature methods

  Nature methods 8 409-412, 2011-04-03

  Nature Publishing Group

Details

• CRID: 1050282810689292928
• NII Article ID: 120002949763
• ISSN: 15487105
• HANDLE: 2433/139494
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN