Zipper-interacting protein kinase modulates canonical Wnt/beta-catenin signaling through interacting with Nemo-like kinase and T-cell factor 4

• Togi, Sumihito

• Ikeda, Sumihito

• Kamitani, Sumihito

• Nakasuji, Sumihito

• Sekine, Sumihito

• Muromoto, Ryuta

• Nanbo, Asuka

• Oritani, Kenji

• Kawai, Taro

• Akira, Shizuo

• Matsuda, Tadashi

抄録

Zipper-interacting protein kinase (ZIPK) is a widely expressed serine/threonine kinase that has been implicated in apoptosis and transcriptional regulation. Here, we identified Nemo-like kinase (NLK) as a novel ZIPK-binding partner, and found that ZIPK regulates NLK-mediated repression of canonical Wnt/β−catenin signaling. Indeed, siRNA-mediated reduction of endogenous ZIPK expression reduced Wnt/β−catenin signaling. Furthermore, ZIPK affected complex formation of NLK-T-cell factor (TCF) 4. Importantly, ZIPK siRNA treatment in human colon carcinoma cells resulted in a reduction of β−catenin/TCF-mediated gene expression and cell growth. These results indicate that ZIPK may serve as a transcriptional regulator of canonical Wnt/β−catenin signaling through interaction with NLK/TCF4.

収録刊行物

• Journal of Biological Chemistry

  Journal of Biological Chemistry 286 (21), 19170-19177, 2011-05

  American Society of Biochemistry and Molecular Biology

詳細情報

• CRID: 1050001339008264704
• NII論文ID: 120003005575
• ISSN: 1083351X
• ISSN: 00219258
• HANDLE: 2115/45474
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles