Origin and function of the stalk-cell vacuole in Dictyostelium.
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抄録
Large vacuoles are characteristic of plant and fungal cells, and their origin has long attracted interest. The cellular slime mould provides a unique opportunity to study the de novo formation of vacuoles because, in its life cycle, a subset of the highly motile animal-like cells (prestalk cells) rapidly develops a single large vacuole and cellulosic cell wall to become plant-like cells (stalk cells). Here we describe the origin and process of vacuole formation using live-imaging of Dictyostelium cells expressing GFP-tagged ammonium transporter A (AmtA-GFP), which was found to reside on the membrane of stalk-cell vacuoles. We show that stalk-cell vacuoles originate from acidic vesicles and autophagosomes, which fuse to form autolysosomes. Their repeated fusion and expansion accompanied by concomitant cell wall formation enable the stalk cells to rapidly develop turgor pressure necessary to make the rigid stalk to hold the spores aloft. Contractile vacuoles, which are rich in H(+)-ATPase as in plant vacuoles, remained separate from these vacuoles. We further argue that AmtA may play an important role in the control of stalk-cell differentiation by modulating the pH of autolysosomes.
収録刊行物
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- Developmental biology
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Developmental biology 352 (1), 48-57, 2011-04-01
Elsevier Inc.
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キーワード
- Dictyostelium
- Stalk-cell vacuole
- Ammonium transporter
- Biological Markers/metabolism
- Cell Proliferation
- Cell Wall/metabolism
- Cell Wall/ultrastructure
- Dictyostelium/cytology
- Dictyostelium/metabolism
- Dictyostelium/ultrastructure
- Green Fluorescent Proteins/metabolism
- Intracellular Membranes/metabolism
- Intracellular Membranes/ultrastructure
- Lysosomes/metabolism
- Lysosomes/ultrastructure
- Phagosomes/metabolism
- Phagosomes/ultrastructure
- Protozoan Proteins/metabolism
- Vacuoles/metabolism
- Vacuoles/ultrastructure
詳細情報 詳細情報について
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- CRID
- 1050001201940780288
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- NII論文ID
- 120003018167
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- NII書誌ID
- AA00627848
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- ISSN
- 00121606
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- HANDLE
- 2433/139914
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles