Effects of Inorganic Mercury on Osteoclasts and Osteoblasts of the Goldfish Scales In Vitro

• 鈴木 信雄

  金澤大学環日本海域環境研究センター臨界実験施設

• 谷内口 孝治

  のと海洋センター

• 早川 和一

  金沢大学大学院自然科学研究科

• 大森 克徳

  宇宙航空研究開発機構

• 高田 耕司

  東京慈恵会医科大学生化学部門

• 田畑 純

  東京医科歯科大学大学院医硬組織構造生物学分野

• 北村 敬一郎

  金沢大学医薬保健学域保健学類

• 遠藤 雅人

  東京海洋大学海洋科学部海洋生物資源学科

• 和田 重人

  富山大学医学部歯科口腔外科

• Srivastav K. Ajai

  Department of Zoology, University of Gorakhpur

• Chowdhury S. Vishwajit

  International Education Center, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University

• 大嶋 雄治

  九州大学大学院農学研究院生物資源環境学府

• 服部 淳彦

  東京医科歯科大学教養部

抄録

The fish scales are the major source of internal calcium requirement due to having a higher internal calcium reservoir than the body skeleton during the periods of drastic calcium demand, such as sexual maturation. Therefore, we developed original in vitro assay system using goldfish scales that contain osteoclasts and osteoblasts, and examined the direct effect of inorganic mercury (HgCl2) on osteoclasts and osteoblasts. In this assay system, we measured the activities of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as respective indicators of each activity in osteoclasts and osteoblasts. TRAP activity in the scales significantly decreased by the treatment of HgCl2 (10-5 to 10-3M) during 6 hrs of incubation. In addition, mRNA expressions of osteoclastic markers: TRAP and cathepsin K significantly decreased compared with control. In our knowledge, this is the first report of a direct effect of inorganic mercury on osteoclasts. On the other hand, ALP activity decreased after exposures of HgCl2 at a concentration of 10-6, 10-5 or 10-4M for 36 and 64 hrs, although its activity did not change after 6 and 18 hrs. The mRNA expression of metallothionein (MT) which is a metal-binding-protein that protects the organism from heavy metal, significantly increased by HgCl2 (10-4M) although insulin-like growth factor-I (osteoblastic marker) was less than those of control scales by treatment with HgCl2 (10-4M). These results suggests that osteoblasts may synthesize MT and protect from mercury until 18 hrs incubation. Thus, the scale in vitro assay system would be a useful means for analysis of heavy metal on bone metabolism.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 56 (1), 47-51, 2011-02

  九州大学大学院農学研究院

キーワード

• inorganic mercury
• in vitro assay
• osteoclasts
• osteoblasts
• scales

詳細情報

• CRID: 1390853649614753664
• NII論文ID: 120003025271
• NII書誌ID: AA00247166
• DOI: 10.5109/19635
• HANDLE: 2324/19635
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles