Trans-differentiation of a Duodenal Phenotype on Duodenal Transplantation of Different Normal Tissues in F344 Rats

• Watanabe, Hiromitsu

• Kominami, Yoko

• Gon, Rina

• Hayashi, Masaomi

• Nishiki, Masayo

• Sasaki, Atsushi

• Shiraishi, Miho

• Ueda, Shinobu

• Ochiya, Takahiro

• Kamiya, Kenji

抄録

The mechanisms regulating stem cell differentiation and self-renewal are largely unknown. Our ultimate goal is to be able to regulate somatic stem cell differentiation and proliferation, In the present study the ability of trans-differentiation was studied when different normal tissue types were transplanted into the duodenum in rats. Pieces of ear (skin), bladder, trachea, diaphragm, pyloric gland. and forestomach from 8-week old GFP-F344 rats were transplanted into the duodenum of F344 rats. Goblet cells with alcian-blue PAS positive mucin and brash border with alkaline phosphatase (ALP) activity appeared in tissues implanted into the duodenum. In addition, GFP-positive duodenal mucosa was observed in all cases by immunohistochemical staining. Moreover, the GFP-positive cells were found to carry the GFP transgene by PCR analysis. indicating that the bladder, trachea. ear (skin), diaphragm, pyloric gland, and farestomach tissues showed a multipotential ability for differentiation. These results indicated that stem cells within tissues have a multipotential ability, trans-differentiating into different organs when transplanted into different environments.

収録刊行物

• Hiroshima Journal of Medical Sciences

  Hiroshima Journal of Medical Sciences 60 (1), 1-6, 2011-03

  Hiroshima University Medical Press

キーワード

• Transplantation
• Hetero-tissues
• Duodenum
• F344 GFP Rats

詳細情報

• CRID: 1050577740962768000
• NII論文ID: 120003221334
• NII書誌ID: AA00664312
• Web Site: http://ir.lib.hiroshima-u.ac.jp/00031592
• Web Site: https://search.jamas.or.jp/link/ui/2012030949
• 本文言語コード: en
• 資料種別: departmental bulletin paper
• データソース種別:
  • IRDB
  • CiNii Articles