Generation of a monoclonal antibody reactive to prefusion myocytes.

• 栗﨑, 知浩

• Masuda, Aki

• Nakagiri, Shiho

• Hayata, Yoshihiro

• Kuhara, Motoki

• Kishi, Yoshiro

• Sehara-Fujisawa, Atsuko

抄録

We established a novel monoclonal antibody, Yaksa that is specific to a subpopulation of myogenic cells. The Yaksa antigen is not expressed on the surface of growing myoblasts but only on a subpopulation of myogenin-positive myocytes. When Yaksa antigen-positive mononucleated cells were freshly prepared from a murine myogenic cell by a cell sorter, they fused with each other and formed multinucleated myotubes shortly after replating while Yaksa antigen-negative cells scarcely generated myotubes. These results suggest that Yaksa could segregate fusion-competent, mononucleated cells from fusion-incompetent cells during muscle differentiation. The Yaksa antigen was also expressed in developing muscle and regenerating muscle in vivo and it was localized at sites of cell-cell contact between mono-nucleated muscle cells and between mono-nucleated muscle cells and myotubes. Thus, Yaksa that marks prefusion myocytes before myotube formation can be a useful tool to elucidate the cellular and molecular mechanisms of myogenic cell fusion.

収録刊行物

• Journal of muscle research and cell motility

  Journal of muscle research and cell motility 32 (1), 31-38, 2011-08

  Springer

キーワード

• Cell fusion
• Myogenesis
• Skeletal muscle
• Prefusion

詳細情報

• CRID: 1050564285674330880
• NII論文ID: 120003324602
• NII書誌ID: AA00258570
• ISSN: 01424319
• HANDLE: 2433/145958
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles