Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus

• Chen, Jiancheng

• Yamada, Shuhei

• Hama, Yoshiki

• Shetty, Ajaya Kumar

• Kobayashi, Takanari

• Oda, Hiroshi

• Seiki, Kosuke

• Kim, Eunmi

• Kimura, Takashi

• Takahashi, Naonori

• Hidari, Kazuya I. P. J.

• Suzuki, Takashi

• Suzuki, Yasuo

• Sugahara, Kazuyuki

抄録

The structure and biological activities of a highly sulfated heparan sulfate (HS) extracted from shrimp (Penaeus brasiliensis) heads were characterized. Structurally the shrimp HS was more heterogenous than heparin, although it is still highly sulfated. The molecular mass of the shrimp HS preparation was determined to be 32.3 kDa by gel filtration HPLC. Analysis by surface plasmon resonance demonstrated that various growth/differentiation factors specifically bound to the shrimp HS with comparable affinity. Notably, the shrimp HS had a greater inhibitory effect against infections by dengue virus type 2 as well as Japanese encephalitis virus than heparin. Experiments on anticoagulant activity indicated that the shrimp HS exhibited significant anti-thrombin activity, but less than the commercial heparin. Hence, the HS preparation from shrimp heads, an industrial waste, is a prospective agent for a variety of clinical applications.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 412 (1), 136-142, 2011-08-19

  Elsevier

キーワード

• Glycosaminoglycans
• Heparan sulfate
• Dengue virus
• Japanese encephalitis virus
• Anticoagulant activity

詳細情報

• CRID: 1050845763939534848
• NII論文ID: 120003370674
• DOI: 10.1016/j.bbrc.2011.07.059
• HANDLE: 2115/47200
• ISSN: 0006291X
• Web Site: https://api.elsevier.com/content/article/PII:S0006291X1101285X?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0006291X1101285X?httpAccept=text/plain
• 本文言語コード: en
• 資料種別: journal article
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