Identification of novel mammalian phospholipids containing threonine, aspartate, and glutamate as the base moiety.

• Omori, Taketo

• Honda, Ai

• 三原, 久明

• 栗原, 達夫

• 江崎, 信芳

抄録

In this study, we showed the occurrence of phosphatidyl-L-threonine (PThr), phosphatidyl-L-aspartate (PAsp), and phosphatidyl-L-glutamate (PGlu) in rat brain. Analyses using an HPLC-ESI-MS and an amino acid analyzer showed the presence of L-threonine, L-aspartate, and L-glutamate in the acid-hydrolysates of phospholipids from porcine cerebrum, rat cerebrum, and rat liver. Results of ESI-MS/MS analyses with neutral loss scanning and product ion scanning suggest the presence of PThr-(18:0, 18:1), PThr-(18:0, 22:6), PAsp-(18:0, 18:1), PAsp-(18:0, 22:6), PGlu-(18:0, 18:1), and PGlu-(18:0, 22:6) in rat brain. This is the first study to identify 2 novel phospholipids, PAsp and PGlu, with a carboxylate-phosphate anhydride bond, in living organisms.

収録刊行物

• Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 879 (29), 3296-3302, 2011-11-01

  Elsevier B.V.

キーワード

• Aspartate
• Glutamate
• Threonine
• Phospholipids
• Mammal
• Carboxylate–phosphate anhydride

詳細情報

• CRID: 1050282810700418560
• NII論文ID: 120003644380
• NII書誌ID: AA11644805
• ISSN: 1873376X
• HANDLE: 2433/151862
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles