Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats

• Ochiai, K.

• Yoshikawa, Y.

• Oonuma, T.

• Tomioka, Y.

• Hashizume, K.

• Morimatsu, M.

抄録

In humans, mutations of the breast cancer susceptibility protein BRCA2 interact with recombinase RAD51 and increase the risk of cancer. This interaction occurs via a series of eight BRC repeat sequences in BRCA2. A mammalian two-hybrid assay using individual BRC repeats demonstrated that all the repeats except BRC6 bind RAD51 strongly (BRC1, 2 and 4), with intermediate strength (BRC8), or weakly (BRC3, 5 and 7). In serial deletion mutation experiments, the binding strengths were increased when the C-terminal BRC repeat was removed from BRC1-8, BRC1-5 and BRC1-3. These results provide an understanding of the basic function of canine BRCA2 and may be helpful to estimate the effect of missense or truncation mutations in canine mammary tumours.

収録刊行物

• The Veterinary Journal

  The Veterinary Journal 190 (2), 293-295, 2011-11

  Elsevier

キーワード

• Canine
• BRCA2
• RAD51

詳細情報

• CRID: 1050282813986310272
• NII論文ID: 120003660622
• DOI: 10.1016/j.tvjl.2010.11.001
• HANDLE: 2115/47982
• ISSN: 10900233
• Web Site: https://api.elsevier.com/content/article/PII:S1090023310003758?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1090023310003758?httpAccept=text/plain
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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