Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines

• Jaswir, Irwandi

• Noviendri, Dedi

• Salleh, Hamzah Mohd.

• Taher, Muhammad

• Miyashita, Kazuo

抄録

Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirteen fatty acids were detected with gas chromatography. However, fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents of P. australis were found to be 2.06, 9.50, 6.37, and 2.83%, respectively. For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid with about 23.97%. Furthermore, data obtained from the methyl thiazolyl tetrazolium (MTT) assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value of 2.45 mM.

収録刊行物

• African Journal of Biotechnology

  African Journal of Biotechnology 10 (81), 18855-18862, 2011-12-16

  Academic Journals

キーワード

• Brown seaweed
• Padina australis
• fucoxanthin
• fatty acid methyl ester
• H1299 cell lines
• human lung cancer
• MTT assay

詳細情報

• CRID: 1050001339010563712
• NII論文ID: 120003837208
• HANDLE: 2115/48392
• ISSN: 16845315
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles