Mechanism for propofol inhibition of Na＋, K＋-ATPase activity in rat brain

• Hase, Yuri

• Deyama, Yoshiaki

• Yoshimura, Yoshitaka

• Suzuki, Kuniaki

• Fukushima, Kazuaki

抄録

Propofol is one of the most widely used intravenous anesthetics, however the mechanism of the anesthetic effect is not fully understood. Na+,K+-ATPase is an enzyme present in all animal cell membranes and plays essential roles for the maintenance of neuronal excitability. There is a report of propofol inhibition of Na+,K+-ATPase activity, but the mechanism is not clearly established. To study the mechanism for propofol inhibition of Na+,K+-ATPase purified from whole brains of rats, the effects of propofol on Na+,K+-ATPase activity, Na+-ATPase, and K+-pNPPase activities, which are partial reactions of Na+,K+-ATPase were examined. Na+,K+-ATPase and Na+-ATPase activities decreased depending on the concentration of propofol, and were completely inhibited at 1.03 mM. Propofol decreased the maximum activity of Na+-, K+-, Mg2+-, and ATPdependent activation of Na+,K+-ATPase activity depending on its concentration, and changed the half maximal concentration for Na+, K+, and ATP, but not for Mg2+. Propofol also decreased the maximum activities of Na+-ATPase and K+-pNPPase, suggesting that propofol inhibits Na+,K+-ATPase activity by affecting the whole reaction process of Na+,K+-ATPase. The inhibition of Na+,K+-ATPase activity by propofol was reversible by dilution of its concentration. These results suggest that propofol reversibly inhibits Na+,K+-ATPase activity in a mixed-type inhibition pattern.

収録刊行物

• 北海道歯学雑誌

  北海道歯学雑誌 32 (2), 147-155, 2012-03

  北海道歯学会

キーワード

• Intravenous anesthetic
• propofol
• Na＋
• K＋-ATPase

詳細情報

• CRID: 1050845763940760960
• NII論文ID: 10030576277
• NII書誌ID: AN00229778
• HANDLE: 2115/48709
• ISSN: 09147063
• Web Site: https://search.jamas.or.jp/link/ui/2013000707
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles