Integrase-independent HIV-1 infection is augmented under conditions of DNA damage and produces a viral reservoir.

• 蝦名, 博貴

• Kanemura, Yuka

• Suzuki, Yasutsugu

• Urata, Kozue

• Misawa, Naoko

• Koyanagi, Yoshio

抄録

HIV-1 possesses a viral protein, integrase (IN), which is necessary for its efficient integration in target cells. However, it has been reported that an IN-defective HIV strain is still capable of integration. Here, we assessed the ability of wild type (WT) HIV-1 to establish infection in the presence of IN inhibitors. We observed a low, yet clear infection of inhibitor-incubated cells infected with WT HIV which was identical to cells infected with IN-deficient HIV, D64A. Furthermore, the IN-independent integration could be enhanced by the pretreatment of cells with DNA-damaging agents suggesting that integration is mediated by a DNA repair system. Moreover, significantly faster viral replication kinetics with augmented viral DNA integration was observed after infection in irradiated cells treated with IN inhibitor compared to nonirradiated cells. Altogether, our results suggest that HIV DNA has integration potential in the presence of an IN inhibitor and may serve as a virus reservoir.

収録刊行物

• Virology

  Virology 427 (1), 44-50, 2012-05-25

  Elsevier Inc.

キーワード

• HIV-1
• Integration
• Integrase inhibitor
• Provirus
• HIV reservoir
• DNA repair

詳細情報

• CRID: 1050845760661962496
• NII論文ID: 120004007474
• NII書誌ID: AA11535118
• ISSN: 00426822
• HANDLE: 2433/154921
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles