Cloning and Characterization of a Laccase Gene from Biotechnologically Important Basidiomycete Cerrena Unicolor

• Janusz Grzegorz

  Department of Biochemistry, Maria Curie–Skłodowska University

• Mazur Andrzej

• Checinsks Aleksandra

  Department of Biochemistry, Maria Curie–Skłodowska University : Environmental Biotechnology Institute, Environmental Science Program, University of Idaho

• Malek Wanda

  Department of Genetics and Microbiology, M. Curie–Skłodowska University

• Rogalski Jerzy

  Department of Biochemistry, Maria Curie–Skłodowska University

• 大賀 祥治

  九州大学大学院農学研究院環境農学部門森林環境科学講座

抄録

The laccase gene lac1 and its corresponding full–length cDNA from white–rot fungi Cerrena unicolor with high yield of laccase production and potential use in electrochemical studies, was cloned and characterized. The 1533 bp full–length cDNA of lac1 encoded a mature laccase protein containing 510 amino acids was preceded by a signal peptide of 20 amino acids. The deduced protein sequence of lac1 shared similarity with other known fungal laccases and contained four copper–binding conservative domains of typical laccase protein An inferred TATA box and several putative CAAT, MRE, XRE consensus sequences were identified in the lac1 promoter region. A number of putative consensus sequences such as those binding AP1, AP2, creA and NIT2 transcription factors, involved in nitrogen and carbon regulation in different fungi, were also present in the promoter region of Cerrena unicolor laccase gene.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 57 (1), 41-49, 2012-02

  九州大学大学院農学研究院

キーワード

• Cerrena unicolor
• laccase
• laccase gene promoter region

詳細情報

• CRID: 1390290699661325056
• NII論文ID: 120004191739
• NII書誌ID: AA00247166
• DOI: 10.5109/22046
• HANDLE: 2324/22046
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles