Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria

• Shen, Jian-Ren

• Kawakami, Keisuke

• Koike, Hiroyuki

抄録

This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.

収録刊行物

• Methods in Molecular Biology

  Methods in Molecular Biology 684 41-51, 2011

  Humana Press

キーワード

• Photosystem II
• Oxygen evolution
• Crystallization
• Membrane proteins
• Ion-exchange chromatography

詳細情報

• CRID: 1050846637354350848
• NII論文ID: 120004622489
• NII書誌ID: AA11067778
• ISSN: 10643745
• Web Site: https://ousar.lib.okayama-u.ac.jp/48703
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles