MOLECULAR DETECTION OF MECT1-MAML2 FUSION GENE IN MUCOEPIDERMOID CARCINOMA WITH ORDINARY AND VARIANT HISTOLOGY: A STUDY USING ARCHIVAL PARAFFIN EMBEDDED TISSUE
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Mucoepidermoid carcinoma (MEC) has been characterized by t (11; 19)(q21; p13). This chromosomal translocation has been recently shown to generate a MECT1- MAML2 fusion gene. MEC can pose diagnostic challenges when they are of high-grade, of variant histologic appearance and occurring in an unusual site. The aim of this study was to evaluate the frequency of the MECT1-MAML2 fusion gene among primary salivary gland MECs and extrasalivary gland MECs, together with some histological variants and its role as a possible diagnostic adjunct, comparing the salivary gland tumors including Warthin's tumor(WT),pleomorphic adenoma(PA),and adenoid cystic carcinoma(ACC). Using a reverse transcription-polymerase chain (RT-PCR)-based approach, we assayed for the MECT1-MAML2 transcript in 39 cases for which paraffin- embedded tumor tissue with adequate RNA was available. These included 19 MECs,10 WTs, five PAs, and five ACCs. The MECT1-MAML2 fusion gene transcript was detected in 16 (84.2%) of 19 MECs. These positive cases included two cases of MEC with WT-like areas,a sclerosing MEC and a clear cell MEC. Three negative cases were high- grade MECs. Two of them were not easy to distinguish from squamous cell carcinoma. The MECT1-MAML2 fusion gene was negative in all cases of WT,PA and ACC. The potential usefulness of MECT1-MAML2 fusion gene expression as a molecular marker in the diagnosis of MEC is supported.
identifier:Journal of Nara Medical Association Vol.62 No.3,4,5 p.69-79
identifier:13450069
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1876
identifier:Journal of Nara Medical Association, 62(3,4,5): 69-79
収録刊行物
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- Journal of Nara Medical Association
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Journal of Nara Medical Association 62 (3,4,5), 69-79, 2011-10-31
奈良医学会
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詳細情報 詳細情報について
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- CRID
- 1050564287448569472
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- NII論文ID
- 120004973704
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- NII書誌ID
- AA11252383
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- ISSN
- 13450069
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- 本文言語コード
- en
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- CiNii Articles