A physical, genetic and functional sequence assembly of the barley genome

• The International Barley Genome Sequencing Consortium

抄録

Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.

収録刊行物

• Nature

  Nature 491 (7426), 711-716, 2012-11-29

  Nature Publishing Group

詳細情報

• CRID: 1572824502528884992
• NII論文ID: 120005121934
• NII書誌ID: AA00752384
• ISSN: 00280836
• Web Site: http://ousar.lib.okayama-u.ac.jp/49077
• 本文言語コード: en
• データソース種別:
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