Hepatic differentiation of embryonic stem cells by murine fetal liver mesenchymal cells.

• 石井, 隆道

• Yasuchika, Kentaro

• Ikai, Iwao

抄録

Hepatocytes derived from embryonic stem cells (ESCs) are a potential cell source for regenerative medicine. However, it has been technically difficult to differentiate ESCs into mature hepatocytes because the definitive growth factors and molecular mechanisms governing hepatocyte differentiation have not yet been well defined. The CD45(-)CD49f(+/-)Thy1(+)gp38(+) mesenchymal cells that reside in murine fetal livers induce hepatic progenitor cells to differentiate into mature hepatocytes by direct cell-cell contact. Utilizing these cells, we employ a two-step procedure for hepatic maturation of ESCs: first, ESCs are differentiated into endodermal cells or hepatic progenitor cells, and second, ESC-derived endodermal cells are matured into functional hepatocytes by coculture with murine fetal liver mesenchymal cells. The ESC-derived hepatocyte-like cells possess hepatic functions, including ammonia removal activity, albumin secretion ability, glycogen synthesis and storage, and cytochrome P450 enzymatic activity.

収録刊行物

• Methods in molecular biology

  Methods in molecular biology 946 469-478, 2013

  Humana Press (Springer Imprint)

キーワード

• embryonic stem cell
• fetal liver
• hepatocyte
• hepatic progenitor cell
• mesenchymal cell
• Thy1
• gp38

詳細情報

• CRID: 1050564285712479616
• NII論文ID: 120005173558
• NII書誌ID: AA11067778
• ISSN: 10643745
• HANDLE: 2433/168971
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles