Enhanced propagation of fish nodaviruses in BF-2 cells persitently infected with snakehead retrovirus (SnRV)

• Nishizawa, Toyohiko

• Kokawa, Yuki

• Wakayama, Takao

• Kinoshita, Shinichi

• Yoshimizu, Mamoru

抄録

Fish nodaviruses are causative agents of viral nervous necrosis causing high mortality in cultured marine fishes around the world. The first successful isolation of fish nodavirus was made with SSN-1 cells, which are persistently infected with snakehead retrovirus (SnRV). In the present study, a BF-2 cell line persistently infected with SnRV (PI-BF-2) was established to evaluate the influence of SnRV on the production of fish nodavirus. The PI-BF-2 cells were slightly more slender than BF-2 cells, but no difference was observed in propagation rate between both cell lines. No difference was observed in production of SnRV between PI-BF-2 and SSN-1 cell lines. Although both PI-BF-2 and BF-2 cell lines showed no cytopathic effect (CPE) after inoculation of striped jack nervous necrosis virus (SJNNV) and redspotted grouper nervous necrosis virus (RGNNV), these fish nodaviruses could be amplified in BF-2 cells, and moreover, production of fish nodaviruses in the PI-BF-2 cell line was more than 40 times higher than in BF-2 cells. Thus, it was concluded that BF-2 cell permissiveness to fish nodaviruses was enhanced by persistent infection with SnRV. Furthermore, homologous cDNA to genomic RNA of SJNNV was detected from both PI-BF-2 and SSN-1 cell lines persistently infected with SnRV. The amount of nodavirus cDNA in SJNNV-inoculated PI-BF-2 cells was clearly lower than that in SJNNV-inoculated SSN-1 cells.

収録刊行物

• Diseases of Aquatic Organisms

  Diseases of Aquatic Organisms 79 (1), 19-25, 2008-03-03

  Inter-Research

キーワード

• Persistent infection
• Fish nodavirus
• SJNNV
• RGNNV
• Retrovirus
• SnRV

詳細情報

• CRID: 1050845763930992256
• NII論文ID: 120005228317
• NII書誌ID: AA10443976
• HANDLE: 2115/52201
• ISSN: 01775103
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles