β-catenin regulates innate and adaptive immunity in mouse liver ischemia-reperfusion injury

• Tsukamoto, Takashi

• Kikukawa, Takashi

• Kurata, Takuro

• Jung, Kwang-Hwan

• Kamo, Naoki

• Demura, Makoto

書誌事項

タイトル別名

• Salt bridge in the conserved His-Asp cluster in Gloeobacter rhodopsin contributes to trimer formation
• Beta-catenin regulates innate and adaptive immunity in mouse liver ischemia-reperfusion injury

抄録

Gloeobacter rhodopsin (GR) is a eubacterial proton pump having a highly conserved histidine near the retinal Schiff base counter-ion, aspartate. Various interactions between His and Asp of the eubacterial proton pump have been reported. Here, we showed the pH-dependent trimer/monomer transition of GR in the presence of dodecyl-β-D-maltoside by size-exclusion chromatography. The pH dependence was closely related to the protonation state of the counter-ion, Asp121. For the H87M mutant, pH dependence disappeared and a monomer became dominant. We concluded that the formation or breaking of the salt bridge between His87 and Asp121 inside the protein changes the quaternary structure.

収録刊行物

• FEBS Letters

  FEBS Letters 587 (4), 322-327, 2013-02-14

  Elsevier B.V.

キーワード

• Microbial rhodopsin
• Histidine-Aspartate cluster
• Quaternary structure
• Protonation
• Size-exclusion chromatography
• Circular dichroism spectroscopy

詳細情報

• CRID: 1050845763943429248
• NII論文ID: 120005228320
• DOI: 10.1002/hep.26100
• HANDLE: 2115/52199
• ISSN: 00145793
• ISSN: 02709139
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fhep.26100
• Web Site: http://onlinelibrary.wiley.com/wol1/doi/10.1002/hep.26100/fullpdf
• 本文言語コード: en
• 資料種別: journal article
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