Characterization of Stimulator of Interferon Genes (STING) Expression in Human Epidermal Keratinocytes

• Nishikawa, Yohei

• Matsuzaki, Yasushi

• Nakano, Hajime

• Sawamura, Daisuke

抄録

The innate immune system is the first line of defense against microbial pathogens, and the production and secretion of interferons （IFNs） plays an important role of the innate response to viral infections. Recently stimulator of interferon genes（ STING） has been identified as an intracellular adaptor, which activates both NF-κB and interferon regulatory factor 3（ IRF3） transcription pathways to induce IFNs. We demonstrated that the STING expression is regu-lated by stimulation of various cytokines in human epidermal keratinocytes （HaCaT）. Especially, IFN-γ induced the STING mRNA expression in a dose-dependent manner. On the oth-er hand, polyinosinicpolycytidylic acid（ poly I:C）, which mimics viral infection, has no effect on the STING expression. STING promoter analysis showed that a gamma-activated se-quence（ GAS） located at －58 to －66 bp is essential for IFN-γ-regulated promoter activity. Moreover, electrophoretic mobility shift assay revealed that STAT1 binds to the GAS element on the STING promoter region, indicating that IFN-γ/JAK/STAT1 signaling is critical for the expression of STING in human epidermal keratinocytes.

弘前医学. 64(Suppl.), 2013, p.S58-S64

収録刊行物

• 弘前医学

  弘前医学 64 (Supplement), S58-S64, 2013-04-02

  弘前大学大学院医学研究科・弘前医学会

キーワード

• 医学
• STING
• IFN-γ
• STAT1
• HaCaT

詳細情報

• CRID: 1050001201682974080
• NII論文ID: 110009616075
• NII書誌ID: AN00211444
• ISSN: 04391721
• HANDLE: 10129/4867
• NDL書誌ID: 024697205
• Web Site: https://hirosaki.repo.nii.ac.jp/records/3777
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024697205
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • NDL
  • CiNii Articles