Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Fulminant Hepatitis in Mice Induced by Concanavalin A

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Stem cell therapy is one of the remarkable treatments for fatal diseases. Mesenchymal stem cells （MSCs） are adult stem cells and detected in various tissues such as bone marrow, umbilical cord blood and adipose tissue. MSCs have some abilities to differentiate into multi-lineage mature cells, modify immune responses and play a role for tissue repairing. The purpose of the present study was to investigate the effect of adipose tissuederived mesenchymal stem cells（ ASCs） against fulminant hepatitis, a life threatening liver injury due to massive destruction of hepatocytes. 　 BALB/c mice were sacrificed and adipose tissues were harvested. ASCs were purified from the tissues and confirmed by characterization of cell surface markers and induction of differentiation into multi-lineage mature cells. Concanavalin A （ConA）, a protein of jackbean, can induce fulminant hepatitis for mice. BALB/c mice were administered lethal or sublethal dose of ConA intravenously and treated by ASCs, phosphate buffered saline（ PBS） or splenocytes （SPLCs）. The survival rates, liver enzymes, cytokines, histopathological changes and localization of ASCs were investigated. 　ASCs could increase the survival rates, inhibit elevation of liver enzymes and cytokines, and attenuate necroses of hepatocytes compared to PBS or SPLCs. Fluorescent stained ASCs were detected in inflammatory liver, but not in normal liver. 　 These findings suggest that ASCs have an ability to improve or attenuate fulminant hepatitis.

弘前医学. 64(Suppl.), 2013, p.S90-S98

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  • 弘前医学

    弘前医学 64 (Supplement), S90-S98, 2013-04-02

    弘前大学大学院医学研究科・弘前医学会

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