Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase for use in cDNA synthesis.

• Konishi, Atsushi

• Shinomura, Mayu

• 保川, 清

抄録

The aim of this study is to explore the advantages of using human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) in cDNA synthesis. Recombinant HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT were produced in an Escherichia coli expression system. In the incorporation of dTTP into poly(rA)-p(dT)(15) (T/P), the K (m) values for dTTP of HIV-1 M RT and HIV-1 O RT were 8 and 12% of that of Moloney murine leukemia virus (MMLV) RT, respectively, and the K (m) values for T/P were 25 and 23% of that of MMLV RT, respectively. Compared with MMLV RT, HIV-1 M RT and HIV-1 O RT were less susceptible to formamide, which is frequently used for cDNA synthesis with a G + C-rich RNA to improve specificity. The high substrate affinity and low susceptibility to formamide of HIV-1 RT might be advantageous for its use in cDNA synthesis.

収録刊行物

• Applied biochemistry and biotechnology

  Applied biochemistry and biotechnology 169 (1), 77-87, 2013-01

  Springer-Verlag

キーワード

• cDNA
• Formamide
• HIV-1
• MMLV
• Reverse transcriptase

詳細情報

• CRID: 1050564285715189888
• NII論文ID: 120005241449
• NII書誌ID: AA10621790
• ISSN: 02732289
• HANDLE: 2433/169949
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles