Lymphocyte-stromal cell interaction induces IL-7 expression by interferon regulatory factors.

• Sekai, Miho

• Tani-Ichi, Shizue

• Yoneyama, Mitsutoshi

• Fujita, Takashi

• Kina, Tatsuo

• 生田, 宏一

抄録

The interaction between lymphocytes and stromal cells plays important roles in coordinated development of early lymphocytes. IL-7 is an essential cytokine for early lymphocyte development produced by stromal cells in the thymus and bone marrow. Although IL-7 is induced by interaction of early lymphocytes and stromal cells, its molecular basis is still unknown. To address this question, we employed co-culture system with an IL-7-dependent pre-B cell line, DW34, and a thymic stromal cell line, TSt-4. Co-culture with DW34 cells enhanced the levels of IL-7 transcripts in TSt-4 cells. Interestingly, the co-culture also induced transcripts of IFN-α and IFN-β but not of IFN-γ. In addition, exogenous IFN-β stimulation increased the levels of IL-7 transcripts in TSt-4 cells. Next, to elucidate the molecular mechanism of IL-7 induction, we analyzed the IL-7 promoter activity by reporter assay. The IL-7 promoter showed specific transcriptional activity in TSt-4 cells. An interferon-stimulated response element (ISRE) in the IL-7 promoter was essential for the induction of IL-7 transcription by both co-culture and IFN-β stimulation. Finally, overexpression of wild-type and dominant-negative forms of interferon regulatory factors (IRFs) activated and repressed, respectively, the IL-7 promoter in TSt-4 cells. Collectively, these results suggested that IRFs activated by lymphocyte adhesion induce IL-7 transcription through ISRE in stromal cells and that type I IFNs may be involved in the activation of IRFs. Thus, this study implied a physiological function of the IFN/IRF signal during lymphocyte development.

収録刊行物

• Molecular immunology

  Molecular immunology 54 (3-4), 378-385, 2013-01

  Elsevier Ltd.

キーワード

• Cytokine
• Cell adhesion
• Transcription factor
• Interferon
• Promoter
• IL-7
• Stromal cell

詳細情報

• CRID: 1050845760694563584
• NII論文ID: 120005242487
• NII書誌ID: AA00307483
• ISSN: 01615890
• HANDLE: 2433/171152
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles