Chemical ligation of oligodeoxynucleotides by X-irradiation and its application to regulation of G-quadruplex formation.

• 田邉, 一仁

• Mizoe, Taiga

• Sugiura, Masaaki

• Okada, Kana

• Nishimoto, Sei-Ichi

抄録

We demonstrated radiolytic ligation of oligodeoxynucleotides (ODNs) possessing disulfide bond and its application to regulation of DNA quadruplex formation. G-rich hexamer ODNs had poor ability to form quadruplex, while X-irradiation of the ODNs induced interstrand exchange reaction at disulfide bond to form ligated 12 mer ODNs, leading to the ready formation of quadruplex due to the entropic effect. Since complexation of the ligated ODNs with hemin in the presence of K(+) showed strong soret band absorption and also catalyzed the H2O2-mediated oxidation of luminol, it appears that the quadruplex formed from ligated ODNs showed a function similar to native DNA quadruplex.

収録刊行物

• Bioorganic & medicinal chemistry letters

  Bioorganic & medicinal chemistry letters 23 (7), 2098-2100, 2013-04

  Elsevier Ltd.

キーワード

• X-Irradiation
• DNA quadruplex
• Chemical ligation
• Chemiluminescence

詳細情報

• CRID: 1050001335767311872
• NII論文ID: 120005244285
• NII書誌ID: AA1079577X
• ISSN: 0960894X
• HANDLE: 2433/173167
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles