Rapid transformation of <italic>Chlamydomonas reinhardtii</italic> without cell-wall removal

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  • Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.

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Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions.

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