Induction of viable gynogenetic progeny using eggs and UV-irradiated sperm from the Chinese tetraploid loach, Misgurnus anguillicaudatus

• Li, Ya-Juan

• Yu, Zhuo

• Zhang, Ming-Zhao

• Qian, Cong

• Abe, Syuiti

• Arai, Katsutoshi

抄録

When eggs from the Chinese tetraploid loach that had 100 chromosomes were fertilized with UV-irradiated sperm, we obtained viable gynogenetic progeny without any additional treatment for the duplication of maternal chromosomes, which survived beyond first feeding towards adult stage of development. Gynogenetic progeny were determined to be diploid since they possessed 50 chromosomes, along with two chromosomes bearing nucleolar organizing regions (NORs), detected by silver nitrate staining (Ag-NORs), chromomycin-A3 (CMA3)-positive sites and fluorescence in situ hybridization (FISH) signals for rDNA loci. In contrast, when gynogens were induced using eggs from diploid loach fertilized by UV-irradiated sperm, but without chromosome doubling, we found that all resultant progeny were non-viable haploid gynogens with 25 chromosomes, along with one NOR-bearing chromosome detected by Ag-NORs, CMA3 and FISH. These observations demonstrate the true genetic tetraploid nature of the Chinese loach possessing 100 chromosomes, and the potential use of this tetraploid as a source of functional diploid gametes for further ploidy manipulation experiments.

収録刊行物

• Aquaculture International

  Aquaculture International 21 (4), 759-768, 2013-08-01

  Springer

キーワード

• Chromosome
• Gametes
• FISH
• Polyploid

詳細情報

• CRID: 1050564288964202880
• NII論文ID: 120005300017
• NII書誌ID: AA11044171
• ISSN: 1573143X
• ISSN: 09676120
• HANDLE: 2115/52956
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles