Distinct Metabolites for Photoreactive l-Phenylalanine Derivatives in Klebsiella sp. CK6 Isolated from Rhizosphere of a Wild Dipterocarp Sapling

• Wang, Lei

• Hisano, Wataru

• Murai, Yuta

• Sakurai, Munenori

• Muto, Yasuyuki

• Ikemoto, Haruka

• Okamoto, Masashi

• Murotani, Takashi

• Isoda, Reika

• Kim, Dongyeop

• Sakihama, Yasuko

• Sitepu, Irnayuli

• Hashidoko, Yasuyuki

• Hatanaka, Yasumaru

• Hashimoto, Makoto

抄録

Abstract: Photoaffinity labeling is a reliable analytical method for biological functionalanalysis. Three major photophores—aryl azide, benzophenone and trifluoromethyldiazirineare utilized in analysis. Photophore-bearing L-phenylalanine derivatives, which are used for biological functional analysis, were inoculated into a Klebsiella sp. isolated from the rhizosphere of a wild dipterocarp sapling in Central Kalimantan, Indonesia, under nitrogenlimiting conditions. The proportions of metabolites were quite distinct for each photophore. These results indicated that photophores affected substrate recognition in rhizobacterial metabolic pathways, and differential photoaffinity labeling could be achieved using different photophore-containing L-phenylalanine derivatives.

収録刊行物

• Molecules

  Molecules 18 (7), 8393-8401, 2013-07

キーワード

• photoaffinity label
• phenylalanine
• tryptophan
• phenyl azide
• benzophenone
• trifluoromethyldiazirine
• metabolites

詳細情報

• CRID: 1050845763944509952
• NII論文ID: 120005301703
• HANDLE: 2115/52962
• ISSN: 14203049
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles