HIRA, a conserved histone chaperone, plays an essential role in low-dose stress response via transcriptional stimulation in fission yeast.
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抄録
Cells that have been pre-exposed to mild stress (priming stress) acquire transient resistance to subsequent severe stress even under different combinations of stresses. This phenomenon is called cross-tolerance. Although it has been reported that cross-tolerance occurs in many organisms, the molecular basis is not clear yet. Here, we identified slm9(+) as a responsible gene for the cross-tolerance in the fission yeast Schizosaccharomyces pombe. Slm9 is a homolog of mammalian HIRA histone chaperone. HIRA forms a conserved complex and gene disruption of other HIRA complex components, Hip1, Hip3, and Hip4, also yielded a cross-tolerance-defective phenotype, indicating that the fission yeast HIRA is involved in the cross-tolerance as a complex. We also revealed that Slm9 was recruited to the stress-responsive gene loci upon stress treatment in an Atf1-dependent manner. The expression of stress-responsive genes under stress conditions was compromised in HIRA disruptants. Consistent with this, Pol II recruitment and nucleosome eviction at these gene loci were impaired in slm9Δ cells. Furthermore, we found that the priming stress enhanced the expression of stress-responsive genes in wild-type cells that were exposed to the severe stress. These observations suggest that HIRA functions in stress response through transcriptional regulation.
収録刊行物
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- The Journal of biological chemistry
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The Journal of biological chemistry 287 (28), 23440-23450, 2012-07-06
American Society for Biochemistry and Molecular Biology
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キーワード
- Stress response
- Cross tolerance
- Histone chaperone
- HIRA
- Fission yeast
- Adaptation, Physiological/genetics
- Adaptation, Physiological/physiology
- Cell Cycle Proteins/genetics
- Cell Cycle Proteins/physiology
- DNA Polymerase II/metabolism
- Dose-Response Relationship, Drug
- Gene Expression Profiling
- Gene Expression Regulation, Fungal
- Histone Chaperones/genetics
- Histone Chaperones/physiology
- Hot Temperature
- Humans
- Hydrogen Peroxide/pharmacology
- Mutation
- Nuclear Proteins/genetics
- Nuclear Proteins/physiology
- Nucleosomes/genetics
- Nucleosomes/metabolism
- Oligonucleotide Array Sequence Analysis
- Oxidants/pharmacology
- Oxidative Stress/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Schizosaccharomyces/drug effects
- Schizosaccharomyces/genetics
- Schizosaccharomyces/growth & development
- Schizosaccharomyces pombe Proteins/genetics
- Schizosaccharomyces pombe Proteins/physiology
- Transcription Factors/genetics
- Transcription Factors/physiology
- Transcription, Genetic
詳細情報 詳細情報について
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- CRID
- 1050564285725969792
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- NII論文ID
- 120005302079
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- NII書誌ID
- AA00251083
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- ISSN
- 00219258
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- HANDLE
- 2433/176347
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles