Phenotype-Based Search of Natural Mutations Related to Hereditary Diseases Existing in a Closed Colony of Mice

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  • KATOH Hideki
    Institute for Experimental Animals, Hamamatsu University School of Medicine Laboratory of Animal Breeding and Genetics, Central Institute for Experimental Animals
  • NISHIKAWA Tetsu
    Institute for Experimental Animals, Hamamatsu University School of Medicine
  • KIMURA Jiro
    Institute for Experimental Animals, Hamamatsu University School of Medicine
  • YAMAUCHI Yumika
    Institute for Experimental Animals, Hamamatsu University School of Medicine
  • TAKABAYASHI Shuji
    Institute for Experimental Animals, Hamamatsu University School of Medicine

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We attempted to detect natural mutations existing in the Jcl:ICR closed colony of mice which is maintained by random mating. We used ordinary genetic backcrosses to efficiently detect recessive mutations carried by individual mice in the colony. Crosses of DBA/2 females and ICR males were performed to obtain F1 mice. Four F1 females randomly selected from each cross were backcrossed to the male parent. More than thirty backcross progeny were obtained from each F1 female by several deliveries. Phenotypes of the backcross progeny were observed macroscopically at about one month of age. As a result, 18 (26.1%) of 69 Jcl:ICR males carried 11 recessive mutation(s). Based on the phenotypes, the tentative names were abnormal kidney, aplasia of eyelids/hind limb digits, circling, dwarfism, heterotaxy, hind limb paralysis, hydrocephalus, rigidity (or rigor), testicular hypoplasia, tremor, and wobbling. The genes responsible for aplasia of eyelids/hind limb digits and dwarfism were each carried by two males, the genes responsible for hydrocephalus and testicular hypoplasia were each carried by three males and the gene responsible for wobbling by four males. It was strongly suggested that the genes shared by several males originated from an identical mutated gene. Surprisingly, male No. 43 had the responsible genes of abnormal kidneys and testicular hypoplasia, and No. 79 had those of dwarfism and tremor. The results obtained in this study suggest that breeders need to be aware of the presence of natural mutations in their colonies.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 59 (2), 183-190, 2010

    公益社団法人 日本実験動物学会

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