Virus-induced expression of retinoic acid inducible gene-I and melanoma differentiation-associated gene 5 in the cochlear sensory epithelium.

• Hayashi, Yushi

• Onomoto, Koji

• Narita, Ryo

• Yoneyama, Mitsutoshi

• Kato, Hiroki

• Nakagawa, Takayuki

• Ito, Juichi

• Taura, Akiko

• 藤田, 尚志

抄録

The inner ear has been regarded as an immunoprivileged site because of isolation by the blood-labyrinthine barrier. Several reports have indicated the existence of immune cells in the inner ear, but there are no reports showing immunocompetence of the cochlear tissue. In this report, we examined the potential involvement of retinoic acid inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5), which are critical for initiating antiviral innate immune responses. We found that RIG-I and MDA5 are expressed in the mouse cochlear sensory epithelium, including Hensen's and Claudius' cells. Exvivo viral infection using Theiler's murine encephalomyelitis virus revealed that the virus replicates in these cells and that protein levels of RIG-I and MDA5 are up-regulated. Furthermore, the critical antiviral transcription factor, interferon (IFN) regulatory factor-3, is activated in the infected cells as judged by its nuclear translocation and the accumulation of type I IFN transcripts. These results strongly suggest that RIG-I and MDA5 participate in innate antiviral responses in cochlear tissue.

収録刊行物

• Microbes and infection

  Microbes and infection 15 (8-9), 592-598, 2013-07

  Elsevier Masson SAS

キーワード

• Innate immunity
• Retinoic acid inducible gene-I family
• Interferon
• Cochlear sensory epithelium
• Supporting cells
• Hensen's and Claudius' cells

詳細情報

• CRID: 1050564285728713088
• NII論文ID: 120005312147
• NII書誌ID: AA11314560
• ISSN: 12864579
• HANDLE: 2433/178059
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles