SIMPLE EXTRACTION OF GAMMA-HYDROXYBUTYRATE IN HUMAN WHOLE BLOOD BY HEADSPACE SOLID-PHASE MICROEXTRACTION

• Kurihara, Rina

• Ishii, Akira

• Watanabe, Kanako

• Kumazawa, Takeshi

• Seno, Hiroshi

• Suzuki, Osamu

• Katsumata, Yoshinao

• Suzuki, Kanako

書誌事項

タイトル別名

• ヘッドスペース固相マイクロ抽出を用いた全血中ガンマヒドロキシ酪酸（GHB）の簡便抽出法

抄録

We have developed a simple method for the extraction of gamma-hydroxybutyrate (GHB) in human whole blood using headspace solid-phase microextraction (SPME). The procedure involves the conversion of GHB to gamma-butyrolactone (GBL) with acid catalysis; gamma-valerolactone (GVL) was used as internal standard (IS). After heating a vial containing a whole blood sample with GHB and IS at 80℃ for 5 min in the presence of H3PO4 solution, a Carboxen/polydimethylsiloxane -coated fiber was exposed to the headspace to allow adsorption of GBL and IS. The fiber needle was then injected into a capillary gas chromatography port. Although the extraction efficiency of GHB was no more than 0.68%, the calibration curve showed good linearity in the range of 10-200μg/ml, and intra-day and inter-day assay coefficients of variation were 3.29 and 4.14%, respectively. The detection limit was about 2μg/ml. The present SPME method for GHB is sensitive enough to be adopted in forensic toxicology and clinical pharmacology .

収録刊行物

• 法中毒

  法中毒 19 (1), 38-45, 2001-01-31

  日本法中毒学会

キーワード

• Solid-phase microextraction
• Headspace method
• Gas chromatography
• Gammahydroxybutyrate
• Gamma-butyrolactone
• Gamma-valerolactone

詳細情報

• CRID: 1050282676648158080
• NII論文ID: 120005314044
• ISSN: 09159606
• HANDLE: 10271/1728
• Web Site: https://hama-med.repo.nii.ac.jp/records/1695
• Web Site: https://search.jamas.or.jp/link/ui/2001142503
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles