新規ネガティブ染色剤を用いた透過型電子顕微鏡による生物試料の観察--分析電子顕微鏡システム利用研究成果、そのXXIII(1)--

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  • Observation for living substances with the transmission electron microscopy using a novel negative staining reagent--Research works accomplished by using Electron Microscope System: XXIII (1)--
  • 新規ネガティブ染色剤を用いた透過型電子顕微鏡による生物試料の観察 : 分析電子顕微鏡システム利用研究成果(その23 1)
  • シンキ ネガティブ センショクザイ オ モチイタ トウカガタ デンシ ケンビキョウ ニ ヨル セイブツ シリョウ ノ カンサツ : ブンセキ デンシ ケンビキョウ システム リヨウ ケンキュウ セイカ(ソノ 23 1)

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Aqueous uranyl acetate has been extensively used as a supeb staining reagent for transmission electron microscopy of biological materials. However, recent regulation of nuclear fuel material severely restricts its use. Since uranyl acetate is hazardous due to biological toxicity and remaining radioactivity, we examined gadolinium acetate (GdAc), a novel staining reagent for negative-staining electron microscopy in comparison with phosphotungstic acid (PTA). As a result, fine TEM images were observed when bacterial cells of Escherichia coli and Thermus thermophilus were respectively stained with I % PTA and 2.5 % GdAc. When GroEL/GroES complexes were stained, the high density sample on the grid was buried in the I % PTA, unless dilution of PTA or GroEUGroES complexes. On the other hand, 2.5 % GdAc stainning made it possible to observe high density sample clearly without dilution. We revealed that Gc!Ac reagents possess excellent capability for staining of bacterial cells (～μm) and GroEL/GroES complexes (～10 nm). GdAc can also be utilized as good negative-staining reagent to study supramolecular architecture ofbiologicall materials.

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