Molecular Characterization of White Leaf Phytoplasma Associated with the Graminae in Myanmar

• Kyu Kyu Win Nang

  Department of Plant Pathology, Yezin Agricultural University

• Kim Young–Hwan

  Laboratory of Forest Resources Management, Division of Agro–environmental Sciences, Department of Forest Environmental Sciences, Faculty of Agriculture, Kyushu University

• Jung Hee-Young

  School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University

• 大賀 祥治

  九州大学大学院生物資源環境科学府森林計画学研究室

抄録

Typical white leaf disease symptoms were observed on Bermuda grass (Cynodon dactylon) in Magway and Nyungoo, and goosegrass (Eleusine indica) in Kyaukpadaung of Myanmar, 2011. Phytoplasma associations with these grasses were determined by polymerase chain reaction analysis with amplified DNA fragment of 1.8 kbp including nearly full–length of 16S rRNA, 16S–23S spacer region and partial of 23S rRNA gene. The 16S rRNA gene sequence analysis resulted that two Bermuda grass white leaf (BGWL) isolates had the highest similarity (99%) with members of ‘Candidatus Phytoplasma cynodontis’. The goosegrass white leaf (GGWL) phytoplasma showed <98.8% identity with 16S rRNA gene sequences of ‘Ca. P. cynodontis’ and sugarcane white leaf phytoplasma (SCWL, AB646271), and shared 99.5% similarity with that of sorghum grassy shoot (SGS, AF509324). Percent homology of 16S–23S spacer region sequences showed that the GGWL isolate was closet (97% identity) to SCWL. Putative restriction site analysis of 16S rRNA gene sequence including spacer region and partial of 23S rRNA gene revealed that the GGWL isolate was distinguished from the BGWL isolate. The phylogenetic analysis proved that the GGWL isolate diverged from the sugarcane white leaf phytoplasmas and closely related with SGS phytoplasma. In addition, the GGWL phytoplasma was distantly related with ‘Ca. P. cynodontis’ and SCWL group. This is the first report on the presence of Bermuda grass white leaf and goose grass white leaf phytoplasmas in Myanmar.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 58 (2), 225-229, 2013-09

  九州大学大学院農学研究院

キーワード

• white leaf phytoplasma
• graminae
• goosegrass

詳細情報

• CRID: 1390572174637294208
• NII論文ID: 120005326646
• NII書誌ID: AA00247166
• DOI: 10.5109/27351
• HANDLE: 2324/27351
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles