c-Myc gene single-strand binding protein-1, MSSP-1, suppresses transcription of alpha-smooth muscle actin gene in chicken visceral smooth muscle cells.
抄録
The expression of alpha-smooth muscle actin is coordinately regulated by positive and negative cis- elements in the promoter region. Although cis -elements and trans -acting factors involved in the positive regulation of the alpha-smooth muscle (alpha-SM) actin gene have been well characterized, details of negative regulation remain unclear. In functional analyses using cultured gizzard smooth muscle cells, we identified a sequence ranging from -238 to -219 in the promoter region as a novel negative element. Mutation and deletion analyses further revealed that a sequence, TATCTTA (-228 to -222), is essential for negative regulation. Gel shift assay and Southwestern blotting indicated that a nuclear protein factor specifically interacts with single- or double-strand DNA including this sequence, and the protein factor displays a highly potent binding to the sense strand DNA. cDNA cloning and gel shift analysis using anti-MSSP-1 antibodies revealed that this protein factor is a chicken homolog of human MSSP-1 (c- myc gene single-strand binding protein-1). In fact, overexpression of MSSP-1 in cultured smooth muscle cells suppresses the promoter activity. These results suggest a novel function of MSSP-1 regarding the transcriptional regulation of alpha-sm actin gene.
収録刊行物
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- Nucleic acids research
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Nucleic acids research 26 (10), 2420-2425, 1998-05-15
Oxford University Press
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キーワード
- Actins/genetics
- Animals
- Cell Extracts
- Cell Nucleus
- Cells, Cultured
- Chick Embryo
- Cloning, Molecular
- DNA/metabolism
- DNA-Binding Proteins/metabolism
- Gene Expression Regulation/genetics
- Gizzard
- Molecular Sequence Data
- Muscle, Smooth/cytology
- Muscle, Smooth/metabolism
- Mutation
- Promoter Regions, Genetic/genetics
- RNA-Binding Proteins
- Recombinant Fusion Proteins
- Transcription, Genetic/genetics
詳細情報 詳細情報について
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- CRID
- 1050001202927731584
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- NII論文ID
- 120005353244
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- HANDLE
- 2115/53971
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- ISSN
- 03051048
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles