Quantitative and Qualitative Involvement of P3N-PIPO in Overcoming Recessive Resistance against Clover yellow vein virus in Pea Carrying cyv1

• Choi, Sun Hee

• Hagiwara-Komoda, Yuka

• Nakahara, Kenji S.

• Atsumi, Go

• Shimada, Ryoko

• Hisa, Yusuke

• Naito, Satoshi

• Uyeda, Ichiro

抄録

In pea carrying cyv1, a recessive gene for resistance to Clover yellow vein virus (ClYVV), ClYVV isolate Cl-no30 was restricted to the initially infected cells, whereas isolate 90-1 Br2 overcame this resistance. We mapped the region responsible for breaking of cyv1-mediated resistance by examining infection of cyv1 pea with chimeric viruses constructed from parts of Cl-no30 and 90-1 Br2. The breaking of resistance was attributed to the P3 cistron, which is known to produce two proteins: P3, from the main open reading frame (ORF), and P3N-PIPO, which has the N-terminal part of P3 fused to amino acids encoded by a small open reading frame (ORF) called PIPO in the +2 reading frame. We introduced point mutations that were synonymous with respect to the P3 protein but nonsynonymous with respect to the P3N-PIPO protein, and vice versa, into the chimeric viruses. Infection of plants with these mutant viruses revealed that both P3 and P3N-PIPO were involved in overcoming cyv1-mediated resistance. Moreover, P3N-PIPO quantitatively affected the virulence of Cl-no30 in cyv1 pea. Additional expression in trans of the P3N-PIPO derived from Cl-no30, using White clover mosaic virus as a vector, enabled Cl-no30 to move to systemic leaves in cyv1 pea. Susceptible pea plants infected with chimeric ClYVV possessing the P3 cistron of 90-1 Br2, and which were therefore virulent toward cyv1 pea, accumulated more P3N-PIPO than did those infected with Cl-no30, suggesting that the higher level of P3N-PIPO in infected cells contributed to the breaking of resistance by 90-1 Br2. This is the first report showing that P3N-PIPO is a virulence determinant in plants resistant to a potyvirus.

収録刊行物

• Journal of Virology

  Journal of Virology 87 (13), 7326-7337, 2013-07

  American Society for Microbiology

キーワード

• Potyvirus
• Frameshift product
• Resistance breaking
• Plant pathology

詳細情報

• CRID: 1050282813991042432
• NII論文ID: 120005372269
• HANDLE: 2115/54599
• ISSN: 0022538X
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles