AHCYL2 (long-IRBIT) as a potential regulator of the electrogenic Na+-HCO3- cotransporter NBCe1-B

• Yamaguchi, Soichiro

• Ishikawa, Toru

抄録

Although AHCYL2 (long-IRBIT) is highly homologous to IRBIT, which regulates ion-transporting proteins including the electrogenic Na+-HCO(3)(-)cotransporter NBCe1-B, its functions are poorly understood. Here, we found that AHCYL2 interacts with NBCe1-B in bovine parotid acinar cells using yeast two-hybrid, immunofluorescence confocal microscopy and co-immunoprecipitation analyses. Whole-cell patch-clamp experiments revealed that co-expression of AHCYL2 reduces the apparent affinity for intracellular Mg2+ in inhibition of NBCe1-B currents specifically in a HCO3- -deficient cellular condition. Our data unveil AHCYL2 as a potential regulator of NBCe1-B in mammalian cells. We propose that cytosolic ionic condition appropriate for AHCYL2 to function might be different from IRBIT. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

収録刊行物

• Febs letters

  Febs letters 588 (5), 672-677, 2014-03-03

  Elsevier

キーワード

• NBCe1-B
• Long-IRBIT
• IRBIT
• Intracellular Mg2+
• Bovine parotid acinar cell
• Whole-cell patch-clamp

詳細情報

• CRID: 1050845763945881856
• NII論文ID: 120005425354
• NII書誌ID: AA00642943
• HANDLE: 2115/55272
• ISSN: 00145793
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles