Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep

• Watanabe, Hitoshi

• Saito, Ryo

• Nakano, Tatsuya

• Takahashi, Hideyuki

• Takahashi, Yu

• Sumiyoshi, Keisuke

• Sato, Katsuyoshi

• Chen, Xiangning

• Okada, Natsumi

• Iwasaki, Shunsuke

• Harjanti, Dian W.

• Sekiguchi, Natsumi

• Sano, Hiroaki

• Kitazawa, Haruki

• Rose, Michael T.

• Ohwada, Shyuichi

• Watanabe, Kouichi

• Aso, Hisashi

• Hubert Vaudry

  editor

抄録

In mice, peripheral 5-HT induces an increase in the plasma concentrations of glucose, insulin and bile acids, and a decrease in plasma triglyceride, NEFA and cholesterol concentrations. However, given the unique characteristics of the metabolism of ruminants relative to monogastric animals, the physiological role of peripheral 5-HT on glucose and lipid metabolism in sheep remains to be established. Therefore, in this study, we investigated the effect of 5-HT on the circulating concentrations of metabolites and insulin using five 5-HT receptor (5HTR) antagonists in sheep. After fasting for 24 h, sheep were intravenously injected with 5-HT, following which-, plasma glucose, insulin, triglyceride and NEFA concentrations were significantly elevated. In contrast, 5-HT did not affect the plasma cholesterol concentration, and it induced a decrease in bile acid concentrations. Increases in plasma glucose and insulin concentrations induced by 5-HT were attenuated by pretreatment with Methysergide, a 5HTR 1, 2 and 7 antagonist. Additionally, decreased plasma bile acid concentrations induced by 5-HT were blocked by pre-treatment with Ketanserin, a 5HTR 2A antagonist. However, none of the 5HTR antagonists inhibited the increase in plasma triglyceride and NEFA levels induced by 5-HT. On the other hand, mRNA expressions of 5HTR1D and 1E were observed in the liver, pancreas and skeletal muscle. These results suggest that there are a number of differences in the physiological functions of peripheral 5-HT with respect to lipid metabolism between mice and sheep, though its effect on glucose metabolism appears to be similar between these species.

収録刊行物

• PLOS ONE

  PLOS ONE 9 (2), e88058-, 2014-02-01

  Public Library of Science

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050282677651960064
• NII論文ID: 120005435471
• DOI: 10.1371/journal.pone.0088058
• ISSN: 19326203
• Web Site: https://iwate-u.repo.nii.ac.jp/records/9386
• Web Site: http://dx.plos.org/10.1371/journal.pone.0088058
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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