Low-frequency dielectric dispersion of bacterial cell suspensions.

• 浅見, 耕司

抄録

Dielectric spectra of Escherichia coli cells suspended in 0.1-10mM NaCl were measured over a frequency range of 10Hz to 10MHz. Low-frequency dielectric dispersion, so-called the α-dispersion, was found below 10kHz in addition to the β-dispersion, due to interfacial polarization, appearing above 100kHz. When the cells were killed by heating at 60°C for 30min, the β-dispersion disappeared completely, whereas the α-dispersion was little influenced. This suggests that the plasma (or inner) membranes of the dead cells are no longer the permeability barrier to small ions, and that the α-dispersion is not related to the membrane potential due to selective membrane permeability of ions. The intensity of the α-dispersion depended on both of the pH and ionic strength of the external medium, supporting the model that the α-dispersion results from the deformation of the ion clouds formed outside and inside the cell wall containing charged residues.

収録刊行物

• Colloids and surfaces. B, Biointerfaces

  Colloids and surfaces. B, Biointerfaces 119 1-5, 2014-07-01

  Elsevier B.V.

キーワード

• Dielectric dispersion
• Bacteria
• Counterion polarization
• Cell death
• Membrane potential
• Cell wall

詳細情報

• CRID: 1050282810770804352
• NII論文ID: 120005439535
• NII書誌ID: AA10919023
• ISSN: 09277765
• HANDLE: 2433/187789
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles