Efficient delivery of immunostimulatory DNA to mouse and human immune cells through the construction of polypod-like structured DNA.

• Uno, Shota

• 西川, 元也

• Mohri, Kohta

• Umeki, Yuka

• Matsuzaki, Noriyuki

• Takahashi, Yuki

• Fujita, Haruyuki

• Kadowaki, Norimitsu

• Takakura, Yoshinobu

抄録

Investigation of mouse macrophage-like RAW264.7 cells showed that the immunostimulatory activity of CpG DNA is increased by formation of polypod-like structured DNA (polypodna), an assembly consisting of three or more oligodeoxynucleotides. To apply CpG polypodna to immunotherapy, its activity was examined in murine dendritic DC2.4 cells, splenic macrophages, and bone marrow-derived dendritic cells (BMDCs). In all cell types, increasing the pod number increased the cellular uptake of DNA and cytokine release. No significant release of cytokines was observed in macrophages lacking Toll-like receptor 9. Similar results were obtained after intradermal injection of polypodna. The polypodna preparations produced significantly higher amounts of interferon α in human peripheral blood mononuclear cells (PBMCs) compared with single-stranded DNA. The conditioned medium of hexapodna-treated human PBMCs effectively inhibited the activity of a hepatitis C virus subgenomic replicon reporter system. These results indicate that polypodna preparations are useful as an immunostimulator.

収録刊行物

• Nanomedicine

  Nanomedicine 10 (4), 765-774, 2014-05

  Elsevier BV

キーワード

• CpG DNA
• Toll-like receptor 9
• Dendritic cell
• Peripheral blood mononuclear cell

詳細情報

• CRID: 1050001335794095104
• NII論文ID: 120005439540
• NII書誌ID: AA11976408
• ISSN: 15499634
• HANDLE: 2433/187794
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles