Dose-rate effect was observed in T98G glioma cells following BNCT.

• 木梨, 友子

• Okumura, Kakuji

• Kubota, Yoshihisa

• Kitajima, Erika

• Okayasu, Ryuichi

• Ono, Koji

• Takahashi, Sentaro

抄録

[Background]It is generally said that low LET radiation produce high dose-rate effect, on the other hand, no significant dose rate effect is observed in high LET radiation. Although high LET radiations are produced in BNCT, little is known about dose-rate effect of BNCT. [Materials and methods]T98G cells, which were tumor cells, were irradiated by neutron mixed beam with BPA. As normal tissue derived cells, Chinese hamster ovary (CHO-K1) cells and DNA double strand breaks (DNA-DSBs) repair deficient cells, xrs5 cells were irradiated by the neutrons (not including BPA). To DNA-DSBs analysis, T98G cells were stained immunochemically with 53BP1 antibody. The number of DNA-DSBs was determined by counting 53BP1 foci. [Results]There was no dose-rate effect in xrs5 cells. D0 difference between 4 cGy/min and 20 cGy/min irradiation were 0.5 and 5.9 at the neutron and gamma-ray irradiation for CHO-K1, and 0.3 at the neutron for T98G cells. D0 difference between 20 cGy/min and 80 cGy/min irradiation for T98G cells were 1.2 and 0.6 at neutron irradiation plus BPA and gamma-ray. The differences between neutron irradiations at the dose rate in T98G cells were supported by not only the cell viability but also 53BP1 foci assay at 24 h following irradiation to monitor DNA-DSBs. [Conclusion]Dose-rate effect of BNCT when T98G cells include 20 ppm BPA was greater than that of gamma-ray irradiation. Moreover, Dose-rate effect of the neutron beam when CHO-K1 cells did not include BPA was less than that of gamma-ray irradiation These present results may suggest the importance of dose-rate effect for more efficient BNCT and the side effect reduction.

収録刊行物

• Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

  Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine 88 81-85, 2014-06

  Elsevier Ltd.

キーワード

• Dose-rate effect
• BNCT
• DNA-DSBs
• Glioma

詳細情報

• CRID: 1050001335794244864
• NII論文ID: 120005456018
• NII書誌ID: AA10874887
• ISSN: 09698043
• HANDLE: 2433/188913
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles