Hyaluronic acid enhances the effect of the PAMPS/PDMAAm double-network hydrogel on chondrogenic differentiation of ATDC5 cells, BMC

• Kitamura, Nobuto

• Kurokawa, Takayuki

• Fukui, Takaaki

• Gong, Jian P.

• Yasuda, Kazunori

書誌事項

タイトル別名

• Hyaluronic acid enhances the effect of the PAMPS/PDMAAm double-network hydrogel on chondrogenic differentiation of ATDC5 cells

抄録

Background: A double-network (DN) gel, which was composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N,N'-dimethyl acrylamide) (PAMPS/PDMAAm), has the potential to induce chondrogenesis both in vitro and in vivo. The present study investigated whether DN gel induced chondrogenic differentiation of ATDC5 cells in a maintenance medium without insulin, and whether supplementation of hyaluronic acid enhanced the chondrogenic differentiation effect of DN gel. Methods: ATDC5 cells were cultured on the DN gel and the polystyrene (PS) dish in maintenance media without insulin for 21 days. Hyaluronic acid having a molecular weight of approximately 800 kDa was supplemented into the medium so that the concentration became 0.01, 0.1, or 1.0 mg/mL. The cultured cells were evaluated using immunocytochemistry for type-2 collagen and real time PCR for gene expression of type-2 collagen, aggrecan, and Sox9 at 7 and 21 days of culture. Results: The cells cultured on the DN gel formed nodules and were stained with an anti-type-2 collagen antibody, and expression of type-2 collagen and aggrecan mRNA was significantly greater on the DN gel than on the PS dish surface (p < 0.05) in the hyaluronic acid-free maintenance medium. Hyaluronic acid supplementation of a high concentration (1.0 mg/mL) significantly enhanced expression of type-2 collagen and aggrecan mRNA in comparison with culture without hyaluronic acid at 21 days (p < 0.05). Conclusions: The DN gel induced chondrogenic differentiation of ATDC5 cells without insulin. This effect was significantly affected by hyaluronic acid, depending on the level of concentration. There is a high possibility that hyaluronic acid plays an important role in the in vivo hyaline cartilage regeneration phenomenon induced by the DN gel.

収録刊行物

• BMC Musculoskeletal Disorders

  BMC Musculoskeletal Disorders 15 222-, 2014-07-06

  Biomed Central

キーワード

• Hyaluronic acid
• Chondrogenic differentiation
• ATDC5
• Double-network hydrogel
• Polymer

詳細情報

• CRID: 1050845763947023616
• NII論文ID: 120005468150
• DOI: 10.1186/1471-2474-15-222
• HANDLE: 2115/56867
• ISSN: 14712474
• Web Site: http://link.springer.com/content/pdf/10.1186/1471-2474-15-222.pdf
• Web Site: http://link.springer.com/article/10.1186/1471-2474-15-222/fulltext.html
• Web Site: http://link.springer.com/content/pdf/10.1186/1471-2474-15-222
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN