Safety Evaluation and Antimutagenic Activity of Bamboo/Wood Vinegars Collected at Different Temperatures

• Lin Han Chien

  Laboratory of Environment Functional Materials, Department of Wood Based Materials and Design, College of Agriculture, National Chiayi University

• Chen Po-Kuang

  Graduate Institute of Forest Products Science and Furniture Engineering, College of Agriculture, National Chiayi University : Master

• Lai Ying-Jang

  Department of Food Science, College of Science and Engineering, National Quemoy University

• Wu She-Ching

  Department of Food Science, College of Life Sciences, National Chiayi University,

• Hwang Gwo-Shyong

  Laboratory of Wood Material Technology, Division of Sustainable Bioresources Science, Department of Agro–environmental Sciences, Faculty of Agriculture, Kyushu University

• 藤本 登留

  九州大学大学院農学研究院環境農学部門

抄録

The biological action of bamboo/wood vinegars collected at different temperatures from the exit of chimney of earthen kiln was evaluated by Salmonella mutagenesis assay, as a safety evaluation (Ames test) and reverse mutation assay (antimutagenic activity). The compounds of the vinegars were analyzed using gas chromatography–mass spectroscopy analysis. The acid, phenol and ketone compounds of bamboo vinegars were 10.65–20.09%, 57.87–65.98% and 10.13–18.76%, and the compounds of wood vinegars were 4.27–14.51%, 50.23–65.03% and 12.93–25.26%, respectively. The vinegars’ safety showed that neither cytotoxicity nor mutagenicity toward Salmonella typhimurium TA98 and TA100 with S9 mix (an external metabolic activation system) at the diluting percent content of vinegars were lower than 20.00% or less, and without the S9 mix was at 33.33% or less. The vinegars at a diluting percent content below 20.00% expressed a dose–dependent inhibitory effect against both 4–nitroquinoline–N–oxide (NQNO), a direct mutagen, and the mutagenicity of aflatoxin B1 (AFB1), an indirect mutagen which requires metabolic activation, in Salmonella typhimurium TA98 and TA100. The inhibition of the vinegars against NQNO and AFB1 toward TA100 was better than those toward TA98. In addition, the main percent of phenol and ketone compounds in the vinegars showed cytotoxicity/mutagenicity and an antimutagenic effect against the strains mentioned above, which may partially account for the biological action of bamboo/wood vinegars.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 59 (2), 359-368, 2014-08-29

  九州大学大学院農学研究院

キーワード

• Ames test
• Antimutagenic Activity
• Bamboo/Wood Vinegar
• Gas Chromatography–Mass
• Spectroscopy
• Safety Evaluation

詳細情報

• CRID: 1390290699661563264
• NII論文ID: 120005472189
• NII書誌ID: AA00247166
• DOI: 10.5109/1467647
• HANDLE: 2324/1467647
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles