Identification of a mammalian vesicular polyamine transporter

• Hiasa, Miki

• Miyaji, Takaaki

• Haruna, Yuka

• Takeuchi, Tomoya

• Harada, Yuika

• Moriyama, Sawako

• Yamamoto, Akitsugu

• Omote, Hiroshi

• Moriyama, Yoshinori

抄録

Spermine and spermidine act as neuromodulators upon binding to the extracellular site(s) of various ionotropic receptors, such as N-methyl-d-aspartate receptors. To gain access to the receptors, polyamines synthesized in neurons and astrocytes are stored in secretory vesicles and released upon depolarization. Although vesicular storage is mediated in an ATP-dependent, reserpine-sensitive fashion, the transporter responsible for this process remains unknown. SLC18B1 is the fourth member of the SLC18 transporter family, which includes vesicular monoamine transporters and vesicular acetylcholine transporter. Proteoliposomes containing purified human SLC18B1 protein actively transport spermine and spermidine by exchange of H+. SLC18B1 protein is predominantly expressed in the hippocampus and is associated with vesicles in astrocytes. SLC18B1 gene knockdown decreased both SLC18B1 protein and spermine/spermidine contents in astrocytes. These results indicated that SLC18B1 encodes a vesicular polyamine transporter (VPAT).

収録刊行物

• Scientific Reports

  Scientific Reports 4 2014-10-30

詳細情報

• CRID: 1050283687827495296
• NII論文ID: 120005477132
• ISSN: 20452322
• Web Site: https://ousar.lib.okayama-u.ac.jp/52902
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles