MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex

• Takahashi, Hidehisa

• Takigawa, Ichigaku

• Watanabe, Masashi

• Anwar, Delnur

• Shibata, Mio

• Tomomori-Sato, Chieri

• Sato, Shigeo

• Ranjan, Amol

• Seidel, Chris W.

• Tsukiyama, Tadasuke

• Mizushima, Wataru

• Hayashi, Masayasu

• Ohkawa, Yasuyuki

• Conaway, Joan W.

• Conaway, Ronald C.

• Hatakeyama, Shigetsugu

抄録

Regulation of transcription elongation by RNA polymerase II (Pol II) is a key regulatory step in gene transcription. Recently, the little elongation complex (LEC)-which contains the transcription elongation factor ELL/EAF-was found to be required for the transcription of Pol II-dependent small nuclear RNA (snRNA) genes. Here we show that the human Mediator subunit MED26 plays a role in the recruitment of LEC to a subset of snRNA genes through direct interaction of EAF and the N-terminal domain (NTD) of MED26. Loss of MED26 in cells decreases the occupancy of LEC at a subset of snRNA genes and results in a reduction in their transcription. Our results suggest that the MED26-NTD functions as a molecular switch in the exchange of TBP-associated factor 7 (TAF7) for LEC to facilitate the transition from initiation to elongation during transcription of a subset of snRNA genes.

収録刊行物

• Nature Communications

  Nature Communications 6 5941-, 2015-01

  Nature Publishing Group

キーワード

• General Physics and Astronomy
• General Biochemistry, Genetics and Molecular Biology
• General Chemistry
• Multidisciplinary

詳細情報

• CRID: 1050564288971538560
• NII論文ID: 120005621881
• DOI: 10.1038/ncomms6941
• HANDLE: 2115/59508
• ISSN: 20411723
• Web Site: https://www.nature.com/articles/ncomms6941.pdf
• Web Site: https://www.nature.com/articles/ncomms6941
• 本文言語コード: en
• 資料種別: journal article
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