Dermal Vγ4(+) γδ T cells possess a migratory potency to the draining lymph nodes and modulate CD8(+) T-cell activity through TNF-α production.

• 中溝, 聡

• 江川, 形平

• Tomura, Michio

• Sakai, Shunsuke

• Tsuchiya, Soken

• Kitoh, Akihiko

• Honda, Tetsuya

• Otsuka, Atsushi

• Nakajima, Saeko

• Dainichi, Teruki

• Tanizaki, Hideaki

• Mitsuyama, Masao

• Sugimoto, Yukihiko

• Kawai, Kazuhiro

• Yoshikai, Yasunobu

• Miyachi, Yoshiki

• Kabashima, Kenji

抄録

A large number of gamma delta T cells (γδ T cells) are located within epithelial tissues including the skin. In mice, epidermal and dermal γδ T cells consist of distinct subsets and have specific roles in cutaneous immune responses. A recent study demonstrated that γδ T cells and cutaneous dendritic cells migrate from the skin to the draining lymph nodes (LNs). However, it remains unclear whether they regulate the antigen-specific immune response within the LNs. Herein, we investigated their properties and role in the LNs using the Mycobacterium bovis bacille Calmette-Guérin (BCG) infection model. In vivo cell labeling analysis revealed that most of the migratory subset comprised dermal Vγ4(+) cells. This population transmigrated from the skin to the LNs in a Gi-coupled chemokine receptor-independent manner. By depleting Vγ4(+) cells, the intranodal expansion of CD8(+) T cell against BCG was significantly attenuated. In addition, in vitro analysis revealed that Vγ4(+) cells produced TNF-α and enhanced IL-12 production by dendritic cells. Taken together, these findings suggest that dermal Vγ4(+) cells are a unique subset that possesses a migratory potency to the skin-draining LNs and enhances the dendritic cell function therein.

収録刊行物

• The Journal of investigative dermatology

  The Journal of investigative dermatology 135 (4), 1007-1015, 2015-01-08

  Nature Publishing Group

キーワード

• Animals
• CD8-Positive T-Lymphocytes/cytology
• Cell Movement
• Cell Proliferation
• Gene Expression Regulation
• Interferon-gamma/metabolism
• Interleukin-12/metabolism
• Langerhans Cells/cytology
• Langerhans Cells/metabolism
• Light
• Lymph Nodes/metabolism
• Lymph Nodes/pathology
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Mycobacterium bovis/metabolism
• Receptors, Antigen, T-Cell, gamma-delta/metabolism
• Skin/immunology
• T-Lymphocyte Subsets/immunology
• Tumor Necrosis Factor-alpha/metabolism

詳細情報

• CRID: 1050564285766911360
• NII論文ID: 120005621945
• NII書誌ID: AA00700733
• ISSN: 0022202X
• HANDLE: 2433/198677
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles