細菌走化性に関わる膜透過系因子の役割

• 山元, 季実子

書誌事項

タイトル別名

• INVOLVEMENT OF MEMBRANE TRANSPORTER COMPONENTS IN BACTERIAL CHEMOTAXIS

抄録

type:Article

Bacterial chemotaxis has been recognized as one of the key properties in pathogenicity and bioremediation. Here I studied on roles of membrane transporter components in chemotaxis of two species, the pathogenic bacterium Vibrio cholerae (classical biotype) strain O395N1 and the pollutant-degrading soil bacterium Burkholderia sp. NK8. First, I found that two V. cholerae proteins, Mlp2 and Mlp3, with similarities to the major amino acid chemoreceptors, mediate responses to L-serine and amino acids with small R groups, respectively. Mlp3 did not bind L-serine but co-expression of a putative serine-binding protein (named SatA) with Mlp3 increased responses to L-serine, suggesting that SatA is a primary receptor and Mlp3 is a transducer to mediate serine response. Second, I found that 3-chlorobenzoate (3CB)-degrading Burkholderia sp. NK8 is attracted to 3CB and its degradation products, 3- and 4-chlorocatechol (3CC, and 4CC), and β-ketoadipate. These tactic abilities were ablished by curing of the megaplasmid pNK8. Taxis to β-ketoadipate was complemented by the introduction of a putative outer membrane porin gene ompNK8 found in pNK8, suggesting that the porin facilitates chemotaxis to β-ketoadipate.

収録刊行物

• 法政大学大学院紀要. 理工学・工学研究科編

  法政大学大学院紀要. 理工学・工学研究科編 56 1-8, 2015-03-24

  法政大学大学院理工学・工学研究科

キーワード

• chemotaxis
• porin
• transducer
• transporter

詳細情報

• CRID: 1050580394713481472
• NII論文ID: 120005623585
• NII書誌ID: AA12677220
• ISSN: 21879923
• Web Site: http://hdl.handle.net/10114/11064
• 本文言語コード: ja
• 資料種別: departmental bulletin paper
• データソース種別:
  • IRDB
  • CiNii Articles