In situ detection and identification of hesperidin crystals in satsuma mandarin (Citrus unshiu) peel cells.

• Inoue, Tsuyoshi

• Yoshinaga, Arata

• Takabe, Keiji

• Yoshioka, Terutaka

• Ogawa, Kazunori

• Sakamoto, Masahiro

• Azuma, Jun-ichi

• 本田, 与一

抄録

[Introduction]Hesperidin, a flavonoid known to have important pharmacological effects, accumulates particularly in the peels of satsuma mandarin (Citrus unshiu). Although histochemical studies have suggested that hesperidin forms crystals in some tissues of the Rutaceae and Umbelliferae, there has been no rigorous in situ detection or identification of hesperidin crystals in C. unshiu. [Objective]To characterise the chemical component of the crystals found in C. unshiu peels using Raman microscopy. [Methods]Sections of C. unshiu peels were made. The distribution and morphology of crystals in the sections were analysed microscopically. Raman microscopy was used to detect hesperidin in the sections directly. [Results]The crystals were more abundant in immature peel and were observed particularly in areas surrounding vascular bundles, around the border between the flavedo and albedo layers and just below the epidermal cells. In the morphological analysis by scanning electron microscopy, needle-shaped crystals aggregated and formed clusters of spherical crystals. Spectra obtained by Raman microscopy of the crystals in the peel sections were consistent with those of the hesperidin standard. [Conclusion]This study showed the detailed distribution of crystals in C. unshiu peels and their main component was identified using Raman microscopy to be hesperidin for the first time.

収録刊行物

• Phytochemical analysis

  Phytochemical analysis 26 (2), 105-110, 2014-11-06

  wiley

キーワード

• In situ detection
• Raman microscopy
• scanning electron microscopy
• albedo
• flavedo
• flavonoid
• fruit peel
• hesperidin
• organic crystals
• Citrus unshiu

詳細情報

• CRID: 1050282810792879104
• NII論文ID: 120005657894
• NII書誌ID: AA10825134
• ISSN: 09580344
• HANDLE: 2433/200179
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles