Effect of AST on age-associated changes of vocal folds in a rat model.

• 水田, 匡信

• 平野, 滋

• Hiwatashi, Nao

• Kobayashi, Toshiki

• Tateya, Ichiro

• Kanemaru, Shin-Ichi

• Nakamura, Tatsuo

• Ito, Juichi

抄録

[Objectives/Hypothesis]Reactive oxygen species (ROS) are associated with aging. Astaxanthin (AST) is a strong antioxidant and has been reported to prevent various ROS-induced diseases. In the current study, we investigated the effect of AST on age-associated histological and mRNA changes of vocal folds. [Study Design]Prospective animal experiment with control. [Methods]Six-month-old Sprague-Dawley rats were fed on a normal powder diet with 0.01% (w/w) AST (aged AST-treated group) or without AST (aged sham-treated group). After 12 months of feeding, the larynges were harvested for histology, immunohistochemical detection of 4-hydroxy-2-nonenal (4-HNE), and quantitative real-time polymerase chain reaction for basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Thirteen-week-old rats were used as a young control group (young group). [Results]The expression of 4-HNE, an oxidative stress marker, significantly increased in the two aged groups compared with the young group. Histological examination showed that the deposition of hyaluronic acid in the lamina propria (LP) was significantly reduced in the aged sham-treated group compared with the young group, but no significant difference was observed between the aged AST-treated group and the young group. There were no significant differences in the mRNA expression of bFGF and HGF between the aged AST-treated group and the young group, although the expression of these genes was significantly reduced in the aged sham-treated group as compared with the young group. [Conclusions]These results suggest that AST has the potential to attenuate age-associated changes of vocal folds.

収録刊行物

• The Laryngoscope

  The Laryngoscope 124 (10), E411-E417, 2014-05-27

  wiley

キーワード

• AST
• age-associated changes
• vocal folds
• reactive oxygen species
• Aging/drug effects
• Aging/metabolism
• Aldehydes/metabolism
• Animals
• Antioxidants/pharmacology
• Chromatography, High Pressure Liquid
• Disease Models, Animal
• Fibrinolytic Agents
• Fibroblast Growth Factor 2/biosynthesis
• Fibroblast Growth Factor 2/genetics
• Follow-Up Studies
• Gene Expression Regulation
• Hepatocyte Growth Factor/biosynthesis
• Hepatocyte Growth Factor/genetics
• Immunohistochemistry
• Male
• Oxidative Stress
• Prospective Studies
• RNA, Messenger/genetics
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species/metabolism
• Real-Time Polymerase Chain Reaction
• Vocal Cord Dysfunction/genetics
• Vocal Cord Dysfunction/metabolism
• Vocal Cord Dysfunction/prevention & control
• Vocal Cords/drug effects
• Vocal Cords/metabolism
• Vocal Cords/pathology
• Xanthophylls/pharmacology

詳細情報

• CRID: 1050845760746315264
• NII論文ID: 120005661538
• NII書誌ID: AA00714953
• ISSN: 0023852X
• HANDLE: 2433/200222
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles