Visualization of the entire differentiation process of murine M cells : suppression of their maturation in cecal patches

• Kimura, Shunsuke

• Yamakami-Kimura, Megumi

• Obata, Yuuki

• Hase, Koji

• Kitamura, Hiroshi

• Ohno, Hiroshi

• Iwanaga, Toshihiko

抄録

The microfold (M) cell residing in the follicle-associated epithelium is a specialized epithelial cell that initiates mucosal immune responses by sampling lumina! antigens. The differentiation process of M cells remains unclear due to limitations of analytical methods. Here we found that M cells were classified into two functionally different subtypes based on the expression of Glycoprotein 2 (GP2) by newly developed image cytometric analysis. GP2-high M cells actively took up luminal microbeads, whereas GP2-negative or low cells scarcely ingested them, even though both subsets equally expressed the other M-cell signature genes, suggesting that GP2-high M cells represent functionally mature M cells. Further, the GP2-high mature M cells were abundant in Peyer's patch but sparse in the cecal patch: this was most likely due to a decrease in the nuclear translocation of RelB, a downstream transcription factor for the receptor activator of nuclear factor-kappa B signaling. Given that murine cecum contains a protrusion of beneficial commensals, the restriction of M-cell activity might contribute to preventing the onset of any excessive immune response to the commensals through decelerating the M-cell-dependent uptake of microorganisms.

収録刊行物

• Mucosal immunology

  Mucosal immunology 8 (3), 650-660, 2015-05

  Nature Publishing Group

詳細情報

• CRID: 1050282813994915968
• NII論文ID: 120005665825
• HANDLE: 2115/60152
• ISSN: 19330219
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles